Detection of avian influenza virus and newcastle disease virus by duplex one step RT PCR
نویسندگان
چکیده
منابع مشابه
Rapid detection and differentiation of Newcastle disease virus isolates by a triple one-step RT-PCR.
A triple one-step RT-PCR was developed to screen and differentiate virulent from avirulent Newcastle disease virus (NDV) isolates. Three sets of oligonucleotides were designed, each specific for amplifying NDV fusion protein gene-specific RNA from virulent, avirulent or all isolates respectively. The sensitivity of one-step RT-PCR was determined using viral RNA extracted from serially diluted N...
متن کاملDetection and differentiation of Newcastle disease virus and influenza virus by using duplex real-time PCR.
Newcastle disease virus (NDV), member of the Paramyxoviridae family and avian influenza virus (AIV), member of the Orthomyxoviridae family, are two main avian pathogens causing serious economic problems in poultry health. Both are enveloped, single-stranded, negative-sense RNA viruses and cause similar symptoms, ranging from sub-clinical infections to severe diseases, including decrease in egg ...
متن کاملDetection of avian influenza virus of H9 subtype in the feces of experimentally infected chickens by RT–PCR
Avian Influenza (AI) is a viral respiratory disease of domestic and wild birds. In the diagnostic laboratory, it is essential to have methods for rapid detection of avian respiratory viruses. Cloacal swabs collected from chickens experimentally infected with H9 subtype AI virus, used in a reverse transcription-polymerase chain reaction (RT-PCR) assay for detection of AI. In infected animals, ...
متن کاملDetection of a novel avian influenza A (H7N9) virus in humans by multiplex one-step real-time RT-PCR assay
BACKGROUND A novel avian influenza A (H7N9) virus emerged in eastern China in February 2013. 413 confirmed human cases, including 157 deaths, have been recorded as of July 31, 2014. METHODS Clinical specimens, including throat swabs, sputum or tracheal aspirates, etc., were obtained from patients exhibiting influenza-like illness (ILIs), especially from those having pneumonia and a history of...
متن کاملSpecific detection of H5N1 avian influenza A virus in field specimens by a one-step RT-PCR assay
BACKGROUND Continuous outbreaks of the highly pathogenic H5N1 avian influenza A in Asia has resulted in an urgent effort to improve current diagnostics to aid containment of the virus and lower the threat of a influenza pandemic. We report here the development of a PCR-based assay that is highly specific for the H5N1 avian influenza A virus. METHODS A one-step reverse-transcription PCR assay ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Open Life Sciences
سال: 2013
ISSN: 2391-5412
DOI: 10.2478/s11535-013-0164-7